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Submitted: 12 Oct 2024
Revision: 05 Dec 2024
Accepted: 20 Jan 2025
ePublished: 31 Jul 2025
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Avicenna J Pharm Res. 2023;4(2): 87-91.
doi: 10.34172/ajpr.1103
  Abstract View: 45
  PDF Download: 44

Original Article

The Effect of Methylene Blue on COVID-19 Patients’ Treatment: A Double-Blind Placebo-Controlled Clinical Trial

Javad Saffari-Chaleshtori 1 ORCID logo, Masoud Hafizi 2 ORCID logo, Ali Baradaran 2 ORCID logo, Keyhan Ghatreh-Samani 3 ORCID logo, Roohollah Mohseni 3 ORCID logo, Akbar Soleimani 2* ORCID logo

1 Department of Clinical Biochemistry, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
2 Clinical Research Development Unit, Hajar Hospital, Shahrekord University of Medical Sciences, Shahrekord, Iran
3 Clinical Biochemistry Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran
*Corresponding Author: Akbar Soleimani, Email: dr.akbarsoleimani@yahoo.com

Abstract

Background: COVID-19 became widespread in 2019 worldwide and affected everyone. Even though it was a disease caused by a new strain of coronavirus with severe symptoms, some drugs could be effective in its treatment. It seems that methylene blue (MB), a drug for treating methemoglobinemia, can be a useful COVID-19 treatment.

Methods: Overall, 60 COVID-19 patients with an O2 saturation of less than 85% and with no underlying diseases were chosen and included in this study. The patients were divided into the placebo control group (n=30) and the MB-treated group (n=30) and received 1 mg/kg of MB for 4 days. Some serum parameters were measured before and after 4 days of treatment. In addition, the O2 saturation was measured every 12 hours. Then, the results were compared with each other.

Results: MB decreased the serum C-reactive protein (P=0.14), lactate dehydrogenase (P=0.08), and creatinine (P=0.12) in the COVID-19 patients non-significantly and increased the serum aspartate aminotransferase (P=0.40) and alanine aminotransferase (P=0.36) parameters in comparison with the control placebo group. However, it had no significant effect on O2 saturation (P=0.52) in COVID-19 patients.

Conclusion: MB, a specific drug for treating methemoglobinemia, had no significant effect on COVID-19 patients.



Please cite this article as follows: Saffari-Chaleshtori J, Hafizi M, Baradaran A, Ghatreh-Samani K, Mohseni R, Soleimani A. The effect of methylene blue on COVID-19 patients’ treatment: a double-blind placebo-controlled clinical trial. Avicenna J Pharm Res 2023;4(2)87-91. doi:10.34172/ajpr.1103
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