en عمومى General گزارش مورد case report <p><strong>Background:</strong> <em>Plantago</em> species have been used in traditional medicine to treat many types of diseases. The detection of apigenin, catalpol, and gallic acid in <em>Plantago lanceolata</em> and <em>Plantago major</em> has been optimized using this protocol.</p> <p><strong>Methods:</strong> The analyses were optimized using the C8 column, acetonitrile, and orthophosphoric acid&ndash;water (1:1%) as mobile phase at a flow rate of 1 mL.min^-1, and a wavelength detector was observed at &lambda; 204 nm.</p> <p><strong>Results:</strong> The limits of detection (LOD) and quantification (LOQ) of the method were &ldquo;0.04 and 0.14 &mu;g/ mL&rdquo;, &ldquo;0.007 and 0.022 &mu;g/mL&rdquo;, as well as &ldquo;0.02 and 0.073 &mu;g/mL&rdquo; for catalpol, apigenin, and gallic acid, respectively. The highest level of apigenin in the dry weight of plants (4.34, and 1.99 &mu;g/mg) was obtained from the spike and aerial parts of <em>P. lanceolata</em> and <em>P. major</em> species. High levels of gallic acid extracted from aerial parts and leaves of both species were 12.85 and 10.11 &mu;g/mg, respectively. The highest amount of catalpol (43.33 and 18.15 &mu;g/mg DW) was obtained from the spike of both <em>Plantago</em> sp. The calibration curves were linear with a correlation coefficient (r>0.9991, 0.9996, and 0.9978).</p> <p><strong>Conclusion:</strong> In sum, the most simple and sensitive method to measure compounds was developed using HPLC, which showed a great validity.</p> Flavonoid, Iridoid glycoside, LOD, LOQ, Phenol 49 54 http://ajpr1.umsha.ac.ir/browse.php?a_code=A-10-2-49&slc_lang=en&sid=1 Samaneh Rahamouz-Haghighi rahamouz_haghighi.s@yahoo.com 1003194753284600237 1003194753284600237 Yes Department of Plant Production and Genetics, Faculty of Agriculture, University of Zanjan, Zanjan, Iran Khadijeh Bagheri 1003194753284600238 1003194753284600238 No Department of Plant Production and Genetics, Faculty of Agriculture, University of Zanjan, Zanjan, Iran Neda Mohsen-Pour 1003194753284600239 1003194753284600239 No Zanjan Pharmaceutical Biotechnology Research Center, Zanjan University of Medical Sciences, Zanjan, Iran Ali Sharafi 1003194753284600240 1003194753284600240 No Zanjan Pharmaceutical Biotechnology Research Center, Zanjan University of Medical Sciences, Zanjan, Iran