en عمومى General گزارش مورد case report <p><strong>Background:</strong> <em>Allium hirtifolium</em> and <em>Satureja khuzestanica</em> are the Iranian endemic plants and proper candidates for antioxidant studies. This study investigated the antioxidant, anti-hemolytic properties, and phytochemical composition in different extracts of <em>A. hirtifolium</em> and <em>S. khuzestanica</em>.</p> <p><strong>Methods:</strong> Hydroalcoholic, methanolic, and n-hexane extracts of <em>A. hirtifolium</em> and <em>S. khuzestanica</em> plants were prepared using soaking and ultrasonic methods. Different plant extracts were evaluated for the presence of secondary metabolites using standard methods based on colorimetric analysis. The antioxidant properties of the compounds were measured by the ferric reducing ability of power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) methods. Finally, the anti-hemolytic effects of the extracts were investigated using the 2,2&rsquo;-Azobis(2-amidinopropane) dihydrochloride (AAPH) methods.</p> <p><strong>Results:</strong> Phytochemical analysis indicated the presence of flavonoids, phenolic acids, glycosides, steroids, and terpenoids in <em>S. khuzestanica</em>, as well as amino acid compounds, flavonoids, saponins, glycosides, and phenols in <em>A. hirtifolium</em>. FRAP assay showed that the methanolic extract of <em>S. khuzestanica</em> and the n-hexane extract of <em>A. hirtifolium</em> have the highest total antioxidant activity. The results of the DPPH assay also demonstrated that the minimum IC₅₀ was related to the hydroalcoholic extract of <em>S. khuzestanica</em> (18.58 &micro;g/mL) and the n-hexane extract of <em>A. hirtifolium</em> (87.95 &micro;g/mL). In general, the extracts of both plants could reduce the percentage of AAPH-induced hemolysis while being significant only in some concentrations (<em>P</em><0.001).</p> <p><strong>Conclusion:</strong> Overall, <em>A. hirtifolium</em> and <em>S. khuzestanica</em> can be used as a herbal supplement in the human diet due to their anti-hemolytic effects.</p> Satureja khuzestanica, Allium hirtifolium, Antioxidant, Anti-hemolytic 1 7 http://ajpr1.umsha.ac.ir/browse.php?a_code=A-10-2-39&slc_lang=en&sid=1 Negar Moradi-Maram 1003194753284600206 1003194753284600206 No Medicinal Plants and Natural Products Research Center, Hamadan University of Medical Sciences, Hamadan, Iran Dara Dastan 1003194753284600207 1003194753284600207 No Medicinal Plants and Natural Products Research Center, Hamadan University of Medical Sciences, Hamadan, Iran Amiri Nili-Ahmadabad amirnili54@gmail.com 1003194753284600208 1003194753284600208 Yes Medicinal Plants and Natural Products Research Center, Hamadan University of Medical Sciences, Hamadan, Iran