en عمومى General پژوهشي Research <p><strong>Background:</strong> Nociception is a common feature of neurological disorders. Certain edible seeds contain bioactive phytochemicals with anti-nociceptive features. <em>Buchholzia coriacea</em> seed is traditionally used in folkloric medicine to manage pain-associated ailments. Hence, this study was conducted to assess the therapeutic properties of THE methanol extract of <em>B. coriacea</em> (MEBC) on nociception and its underlying mechanisms in Swiss mice.</p> <p><strong>Methods:</strong> <em>B. coriacea</em> seeds were purchased from the indigenous market in Oje, Ibadan, and authenticated at the Forest Herbarium Ibadan (FHI: 110572). The seeds were pulverized and cold-extracted with methanol. Forty-five male Swiss mice (23-27 g) were divided into nine treatment groups (n=5): control (10 mL/kg), MEBC (50, 100, 200 mg/kg, p.o.), and indomethacin (10 mg/kg, p.o.). Anti-nociceptive activity was evaluated one hour post-administration using the acetic acid-induced writhing test (AAWT), formalin-induced paw licking test (FPLT), and tail flick test (TFT).</p> <p><strong>Results:</strong> The gas chromatography&ndash;mass spectrometry (GC-MS) analysis revealed 31 compounds, with linoleic acid being the most abundant. In the mechanistic study, mice were pre-treated with naloxone (1 mg/kg), atropine (2 mg/kg), propranolol (10 mg/kg), or haloperidol (1.5 mg/kg), 15 minutes before MEBC (200 mg/kg) administration. MEBC (50, 100, 200 mg/kg) significantly reduced writhing in the AAWT, inflammatory pain in the FPLT, and increased latency in the TFT. The opiate blocker naloxone (1 mg/kg), cholinergic antagonist atropine (2 mg/kg), beta-adrenergic blocker propranolol (10 mg/kg), and dopamine D2 receptor antagonist haloperidol (1.5 mg/kg) were administered and significantly reversed the anti-nociception effect of MEBC when compared with the 200 mg/kg treatment in the TFT model.</p> <p><strong>Conclusion:</strong> The findings from this study demonstrate that <em>B. coriacea</em> inhibits nociception, which is attributed to the synergistic activity of its bioactive compounds as indicated by the GS-MC. The mechanisms of action are mediate via central nervous system pathways.</p> Nociception, Buchholzia coriacea, Abdominal writhing, Latency period, Naloxone, Haloperidol 74 86 http://ajpr1.umsha.ac.ir/browse.php?a_code=A-10-2-17&slc_lang=en&sid=1 Lawrence D. Adedayo delawbaba@gmail.com 1003194753284600121 1003194753284600121 Yes Neurophysiology Research Group, Physiology Programme, Bowen University, Iwo, Nigeria Nimedia G. Aitokhuehi 1003194753284600122 1003194753284600122 No Neurosciences Unit, Physiology Department, University of Ibadan, Nigeria Abayomi M. Ajayi 1003194753284600123 1003194753284600123 No Pharmacology and Therapeutics Department, University of Ibadan, Nigeria Olubayode Bamidele 1003194753284600124 1003194753284600124 No Neurophysiology Research Group, Physiology Programme, Bowen University, Iwo, Nigeria Adeshina O. Adekeye 1003194753284600125 1003194753284600125 No Department of Anatomy, Afe Babalola University, Ado-Ekiti, Nigeria Samuel A. Onasanwo 1003194753284600126 1003194753284600126 No Neurosciences Unit, Physiology Department, University of Ibadan, Nigeria