Avicenna Journal of Pharmaceutical Research

en Development, Statistical Optimization and In Vitro/ In Vivo Characterization of an Intelligent Hydrogel Containing Methylated N-(4-N, N-Dimethylaminobenzyl) Chitosan for Glucose-Responsive Insulin Delivery تخصصي Special پژوهشي Research Background: The present study aimed to develop an intelligent, glucose-responsive hydrogel composed of methylated N-(4-N,N-dimethylaminobenzyl) chitosan as a quaternized and aromatized derivative of chitosan. Methods: In this study, a cross-linked hydrogel, incorporating boric acid (BA), was prepared from the synthesized chitosan derivative. The preparation of hydrogel was statistically optimized using box-behnken response surface methodology. The chemical structure and morphology of the optimized hydrogel were examined using FT-IR spectroscopy and scanning electron microscopy, respectively. Furthermore, the glucose-responsive behavior of the prepared hdyrogel was evaluated by in vitro release studies. Finally, the efficacy of the prepared hydrogel in glucose-responsive insulin delivery was studied in vivo by implementation of the hydrogels in male Wistar rats and their blood glucose levels were evaluated in the pre-determined time intervals. Results: The optimized hydrogel with proper characteristics was prepared. Following 480 min of incubation, the in vitro release study demonstrated 26.1 ± 1.84%, 52.7 ± 3.11% and 78.9 ± 3.85% of cumulative insulin release in glucose-free-, glucose 3%- and glucose 5%- phosphate buffer, respectively. Finally, the obtained in vivo results demonstrated 1.5-fold reduction in blood glucose level in diabetic animals compared to non-diabetic ones. Conclusion: The findings obtained suggests that the hydrogel can efficiently pose glucose-responsive properties for insulin delivery.   Boric acid, Glucose-responsive, Hydrogel, Methylated N-(4-N, N-dimethylaminobenzyl) Chitosan, Poly vinyl alcohol (PVA) 27 46 http://ajpr.umsha.ac.ir/browse.php?a_code=A-10-2-84&slc_lang=en&sid=1 Arman Kiani No 1. Department of Pharmaceutics, School of Pharmacy, Hamadan University of Medical Sciences, Hamadan, Iran Amir Larki Harchegani No 2. Department of Pharmacology and Toxicology, School of Pharmacy, Hamadan University of Medical Sciences, Hamadan, Iran Rasool Haddadi No 2. Department of Pharmacology and Toxicology, School of Pharmacy, Hamadan University of Medical Sciences, Hamadan, Iran Reza Mahjub r.mahjoub@umsha.ac.ir Yes 1. Department of Pharmaceutics, School of Pharmacy, Hamadan University of Medical Sciences, Hamadan, Iran