Avicenna Journal of Pharmaceutical Research
Avicenna Journal of Pharmaceutical Research
Avicenna J Pharma Res
Medical Sciences
http://ajpr.umsha.ac.ir
1
admin
2717-1884
10.53208
en
jalali
1404
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gregorian
2025
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online
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Determination of Total Flavonoid and Phenolic Contents and In Vitro Evaluation of Antioxidant Effects of Rosa persica
تخصصي
Special
پژوهشي
Research
<span style="font-size:11pt"><span style="text-autospace:none"><span style="line-height:115%"><span style="font-family:"Times New Roman","serif""><span style="letter-spacing:-0.2pt"><b><span style="font-size:10.0pt"><span style="line-height:115%"><span style="color:black">Background:<i> </i></span></span></span></b><i><span style="font-size:10.0pt"><span style="line-height:115%"><span style="color:black">Rosa persica </span></span></span></i><span style="font-size:10.0pt"><span style="line-height:115%"><span style="color:black">(RP), a member of the <i>Rosaceae </i>family, is known for its high phenolic content. These compounds reportedly possess antiradical and antioxidant activities. This study aimed to investigate the total flavonoid and phenolic contents of three parts of RP and their potential antioxidant </span></span></span><span style="font-size:10.0pt"><span style="line-height:115%">and antiradical <span style="color:black">activities using two methods (</span>ferric reducing ability of plasma<b> </b><span style="color:black">[FRAP] and 1,1-diphenyl-2-picrylhydrazyl [DPPH] assays).</span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-autospace:none"><span style="line-height:115%"><span style="font-family:"Times New Roman","serif""><span style="letter-spacing:-0.2pt"><span class="A2" style="color:black"><b><span style="font-size:10.0pt"><span style="line-height:115%">Methods:</span></span></b></span><span class="A2" style="color:black"><span style="font-size:10.0pt"><span style="line-height:115%"> Flowers, fruits, and seeds of RP</span></span></span><span style="font-size:10.0pt"><span style="line-height:115%"><span style="color:black"> were collected from Hamedan Province, Iran, and the hydroalcoholic extracts were prepared from each part separately. Then, the total flavonoid and phenolic contents of the extracts were determined, and the antioxidant activities were evaluated using the FRAP and DPPH assays.</span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-autospace:none"><span style="line-height:115%"><span style="font-family:"Times New Roman","serif""><span style="letter-spacing:-0.2pt"><b><span style="font-size:10.0pt"><span style="line-height:115%"><span style="color:black">Results:</span></span></span></b><span style="font-size:10.0pt"><span style="line-height:115%"><span style="color:black"> The total phenolic content of the fruit, seed, and flower extracts was 137.56 ± 9.98, 191.79 ± 20.51, and 203.15 ± 4.58 mg of gallic acid equivalent/1 g of hydroalcoholic extract, respectively. The total flavonoid content of the fruit, seed, and flower extracts was 74.72 ± 15.60, 70.00 ± 16.12, and 120.53 ± 0.00 mg of rutin/1 g of hydroalcoholic extract, respectively. The FRAP assay results of the fruit, seed, and flower extracts were 3.95 ± 0.49, 13.92 ± 1.78, and 11.32 ± 173 µmol of ferrous sulfate.7H<sub>2</sub>O/1 g of hydroalcoholic extract, respectively. The IC<sub>50</sub> values</span></span></span><span style="font-size:10.0pt"><span style="line-height:115%"> <span style="color:black">of the extracts in the DPPH assay were 899.8, 45.83, and 70.75 µg/mL. </span></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:115%"><span style="font-family:"Times New Roman","serif""><b><span style="color:black">Conclusion:</span></b><span style="color:black"> The results suggest that </span>extracts from different parts of RP are rich sources of flavonoids and phenolic compounds as natural antioxidants and can be used to prevent deleterious effects caused<span class="A2" style="color:black"><span style="font-size:11.0pt"><span style="line-height:115%"> by </span></span></span>free radical<span class="A2" style="color:black"><span style="font-size:11.0pt"><span style="line-height:115%">s.</span></span></span></span></span></span><br>
Antioxidant activity, Flavonoid content, In vitro, Oxidative stress, Phenolic content, Rosaceae
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http://ajpr.umsha.ac.ir/browse.php?a_code=A-10-2-81&slc_lang=en&sid=1
Amir
Nili-Ahmadabadi