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Submitted: 10 Jul 2021
Accepted: 03 Aug 2021
ePublished: 20 Aug 2021
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Avicenna J Pharm Res. 2021;2: 20-24.
doi: 10.34172/ajpr.2021.04
  Abstract View: 265
  PDF Download: 187

Research Article

Effect of 50-Hz Magnetic Fields on Argyrophilic Nucleolar Organizer Regions in Bone Marrow Cells

Mohammad Rafiee 1 ORCID logo, Mohammad Abbasi 2, Mohammad Yousef Alikhani 3, Safoora Nikzad 4, Alireza Zamani 5* ORCID logo

1 Department of Medical Laboratory Sciences, School of Paramedicine, Hamadan University of Medical Sciences, Hamadan, Iran
2 Department of Internal Medicine, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
3 Department of Microbiology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
4 Department of Medical Physics, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
5 Department of Immunology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
*Corresponding Author: *Corresponding author: Alireza Zamani, Department of Immunology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran Tel: +98 813-8276295-8 ext. 442, Fax: +98 813-8276299, Email: , Email: a_zamani@umsha.ac.ir

Abstract

Background: Exposure to an increasing amount of extremely low-frequency magnetic fields (MFs) causes some adverse effects. Considering a direct association between the sizes and numbers of argyrophilic nucleolar organizer regions (AgNORs) and cell proliferation, this study was conducted to evaluate the effect of 50-Hz MFs on the AgNORs of bone marrow (BM) cells.

Methods: Eighty male rats were randomly divided into four exposed groups and one control group. The exposed groups received 50-Hz MFs with magnetic flux densities of 1, 100, 500, and 2000 µT two hours a day for two months. BM cells were aspirated from sacrificed rats’ femoral bones, smeared on glass slides, and then stained with silver nitrate for AgNORs. The area (AA), length (AL), and number (AN) of AgNORs were calculated by a microscope equipped with a camera and Scion Image software in 100 BM cells of each rat. The mean of AA, AL, and AN was computed for each group.

Results: AA, AL, and AN significantly reduced in the 1 µT group compared with 2000 µT and control groups. Eventually, there was a nonlinear relationship between the effect of 50-Hz MFs and magnetic flux densities.

Conclusion: Overall, 50-Hz MFs with a magnetic flux density of 1 µT reduced AgNORs in BM cells. However, 100, 500, and 2000 µT did not affect AgNORs. Therefore, 50-Hz MFs with low density may suppress BM cell proliferation.


Keywords: Bone marrow, Argyrophilic nucleolar organizer regions, Magnetic fields


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